ELISA/assay

Porcine Cytochrome C ELISA Kit

MBS743668

48-Strip-Wells

440 USD

ELISA Kit

Porcine Cytochrome C ELISA Kit

[Cytochrome C]

[cytochrome c; Cytochrome c; cytochrome c; cytochrome c, somatic]

[Cyt c]

[CYCS; CYCS; CYC; HCS; THC4; CYC]

CYC_HUMAN

Porcine

This assay has high sensitivity and excellent specificity for detection of Cy.C. No significant cross-reactivity or interference between Cy.C and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Cy.C and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This Cy.C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine Cy.C. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the assay: Cy.C ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Cy.C antibody and an Cy.C-HRP conjugate. The assay sample and buffer are incubated together with Cy.C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Cy.C concentration since Cy.C from samples and Cy.C-HRP conjugate compete for the anti-Cy.C antibody binding site. Since the number of sites is limited, as more sites are occupied by Cy.C from the sample, fewer sites are left to bind Cy.C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Cy.C concentration in each sample is interpolated from this standard curve.

11128019

NP_061820.1

NM_018947.5

11,749 Da

AGE/RAGE Pathway (698754); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (105672); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470)

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family. Protein type: Protein phosphatase, Ser/Thr (non-receptor); Apoptosis; Oxidoreductase; Mitochondrial. Chromosomal Location of Human Ortholog: 7p15.3. Cellular Component: protein phosphatase type 2A complex; mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; nucleus; cytosol. Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; protein binding; iron ion binding; heme binding; protein serine/threonine phosphatase activity. Biological Process: mitochondrion organization and biogenesis; cellular metabolic process; response to reactive oxygen species; dephosphorylation; apoptosis; organelle organization and biogenesis; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; caspase activation via cytochrome c; DNA fragmentation during apoptosis; transmembrane transport; mitochondrial electron transport, ubiquinol to cytochrome c. Disease: Thrombocytopenia 4

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415