product summary
request information :
company name :
MyBioSource
product type :
ELISA/assay
product name :
Bovine Leptin ELISA Kit
catalog :
MBS743525
quantity :
96 Strip Wells
price :
675 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS743525
products type :
ELISA Kit
products full name :
Bovine Leptin ELISA Kit
products short name :
Leptin
other names :
leptin; leptin; obesity homolog; leptin<; N/A
products gene name :
Leptin
other gene names :
lep; ob; xlep; N/A
reactivity :
Bovine
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This Leptin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine Leptin. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
other info2 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This Leptin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine Leptin. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Signal Transduction
products description :
Principle of the Assay: Leptin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Leptin antibody and an Leptin-HRP conjugate. The assay sample and buffer are incubated together with Leptin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Leptin concentration since Leptin from samples and Leptin-HRP conjugate compete for the anti-Leptin antibody binding site. Since the number of sites is limited, as more sites are occupied by Leptin from the sample, fewer sites are left to bind Leptin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Leptin concentration in each sample is interpolated from this standard curve.
ncbi gi num :
148233267
ncbi acc num :
NP_001089183.1
ncbi gb acc num :
NM_001095714.1
ncbi pathways :
Adipocytokine Signaling Pathway 119298!!Adipocytokine Signaling Pathway 505!!Cytokine-cytokine Receptor Interaction Pathway 119282!!Cytokine-cytokine Receptor Interaction Pathway 460!!Jak-STAT Signaling Pathway 84661!!Jak-STAT Signaling Pathway 488!!Neuroactive Ligand-receptor Interaction Pathway 119283!!Neuroactive Ligand-receptor Interaction Pathway 462
size :
96 Strip Wells
price :
675 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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