catalog number :
MBS743478
products type :
ELISA Kit
products full name :
Human Hyperglycosylated Human chorionic gonadotropin ELISA Kit
products short name :
Hyperglycosylated Human chorionic gonadotropin
products gene name :
hCG H
specificity :
This assay has high sensitivity and excellent specificity for detection of HhCG. No significant cross-reactivity or interference between HhCG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HhCG and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Sandwich. Sensitivity: 1.0 nmol/L.
products description :
Intended Uses: This HhCG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HhCG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HhCG ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HhCG. Standards or samples are then added to the microtiter plate wells and HhCG if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HhCG present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HhCG are added to each well to “sandwich” the HhCG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HhCG and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HhCG concentration in each sample is interpolated from this standard curve.