ELISA/assay

Rat Prostacyclin ELISA Kit

MBS743424

48-Strip-Wells

470 USD

ELISA Kit

Rat Prostacyclin ELISA Kit

Prostacyclin

prostacyclin receptor; Prostacyclin receptor; prostacyclin receptor; PGI receptor; PGI2 receptor; prostanoid IP receptor; prostaglandin I2 receptor; prostaglandin I2 (prostacyclin) receptor (IP); Prostaglandin I2 receptor; PGI receptor; PGI2 receptor; Prostanoid IP receptor

PGI2

PTGIR; PTGIR; IP; PRIPR; PRIPR; PGI receptor; PGI2 receptor

PI2R_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This PGI2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PGI2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PGI2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGI2 antibody and an PGI2-HRP conjugate. The assay sample and buffer are incubated together with PGI2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGI2 concentration since PGI2 from samples and PGI2-HRP conjugate compete for the anti-PGI2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PGI2 from the sample, fewer sites are left to bind PGI2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGI2 concentration in each sample is interpolated from this standard curve.

4506263

NP_000951.1

NM_000960.3

40,956 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Eicosanoid Ligand-binding Receptors Pathway (106370); Endothelin Pathway (672453); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Hemostasis Pathway (106028); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

The protein encoded by this gene is a member of the G-protein coupled receptor family 1 and has been shown to be a receptor for prostacyclin. Prostacyclin, the major product of cyclooxygenase in macrovascular endothelium, elicits a potent vasodilation and inhibition of platelet aggregation through binding to this receptor. [provided by RefSeq, Jul 2008]

PTGIR: Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19q13.3. Cellular Component: integral to plasma membrane; plasma membrane; cytosol. Molecular Function: G-protein coupled receptor activity; guanyl-nucleotide exchange factor activity. Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; cell-cell signaling; negative regulation of smooth muscle cell proliferation; positive regulation of cAMP biosynthetic process; response to lipopolysaccharide; blood coagulation; G-protein signaling, adenylate cyclase activating pathway; positive regulation of GTPase activity

48-Strip-Wells

470 USD

96-Strip-Wells

675