ELISA/assay

Monkey B-Cell Activation Factor ELISA Kit

MBS743408

48-Strip-Wells

470 USD

ELISA Kit

Monkey B-Cell Activation Factor ELISA Kit

B-Cell Activation Factor

Bcl2; Bcl2; zBlp2; B-cell leukemia/lymphoma 2; B-cell leukemia/lymphoma 2a; B-cell CLL/lymphoma 2a

BAFF

bcl2a; bcl2

Monkey

This assay has high sensitivity and excellent specificity for detection of BAFF. No significant cross-reactivity or interference between BAFF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BAFF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL

Cell Biology

Intended Uses: This BAFF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey BAFF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: BAFF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BAFF. Standards or samples are then added to the microtiter plate wells and BAFF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BAFF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BAFF are added to each well to "sandwich" the BAFF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BAFF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAFF concentration in each sample is interpolated from this standard curve.

71834668

NP_001025424.1

NM_001030253.2

Adrenergic Signaling In Cardiomyocytes Pathway (908262); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Apoptosis Pathway (921011); Apoptosis Pathway (83637); Apoptosis Pathway (470); B Cell Receptor Signaling Pathway (198947); FAS Pathway And Stress Induction Of HSP Regulation (198973); Focal Adhesion Pathway (119265); Focal Adhesion Pathway (478); IL-2 Signaling Pathway (198953)

48-Strip-Wells

470 USD

96-Strip-Wells

675