ELISA/assay

Bovine Neutrophil Gelatinase Associated Lipocalin/Lipocalin-2 ELISA Kit

MBS743380

48-Strip-Wells

470 USD

ELISA Kit

Bovine Neutrophil Gelatinase Associated Lipocalin/Lipocalin-2 ELISA Kit

Neutrophil Gelatinase Associated Lipocalin/Lipocalin-2

neutrophil gelatinase-associated lipocalin; Neutrophil gelatinase-associated lipocalin; neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; oncogene 24p3; siderocalin LCN2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; lipocalin 2; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25

NGAL/LCN2

LCN2; LCN2; 24p3; MSFI; NGAL; HNL; NGAL; NGAL

NGAL_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of NGAL. No significant cross-reactivity or interference between NGAL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NGAL and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Cell Biology

Intended Uses This NGAL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine NGAL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: NGAL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NGAL antibody and an NGAL-HRP conjugate. The assay sample and buffer are incubated together with NGAL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NGAL concentration since NGAL from samples and NGAL-HRP conjugate compete for the anti-NGAL antibody binding site. Since the number of sites is limited, as more sites are occupied by NGAL from the sample, fewer sites are left to bind NGAL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NGAL concentration in each sample is interpolated from this standard curve.

38455402

NP_005555.2

NM_005564.3

22,457 Da

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding. Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; siderophore transport; positive regulation of cell projection organization and biogenesis; innate immune response; ion transport; response to herbicide; transmembrane transport

48-Strip-Wells

470 USD

96-Strip-Wells

675