ELISA/assay

Monkey beta2 Microglobulin ELISA Kit

MBS743367

48-Strip-Wells

440 USD

ELISA Kit

Monkey beta2 Microglobulin ELISA Kit

[beta2 Microglobulin]

[Monkey b2 Microglobulin ELISA Kit]

[beta-2-microglobulin; beta-2-microglobulin; beta2 microglobulin]

[beta2-MG]

[b2-MG]

[B2m]

Monkey

This assay has high sensitivity and excellent specificity for detection of beta2MG. No significant cross-reactivity or interference between beta2MG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between beta2MG and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 2.5-50?g/mL/. Sensitivity: 0.1?g/mL/

Cell Biology

Intended Uses This beta2MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey beta2MG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: beta2MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-beta2MG antibody and an beta2MG-HRP conjugate. The assay sample and buffer are incubated together with beta2MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the beta2MG concentration since beta2MG from samples and beta2MG-HRP conjugate compete for the anti-beta2MG antibody binding site. Since the number of sites is limited, as more sites are occupied by beta2MG from the sample, fewer sites are left to bind beta2MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The beta2MG concentration in each sample is interpolated from this standard curve.

290491208

NP_001166327.1

NM_001172856.1

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415