catalog number :
MBS743317
products type :
ELISA Kit
products full name :
Canine Thrombin-antithrombin complex ELISA Kit
products short name :
Thrombin-antithrombin complex
other names :
tat, partial; Protein Tat
uniprot entry name :
E2FMT9_9HIV1
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 ng/mL
other info2 :
Intended Uses: This TAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TAT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the Assay: TAT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microliter plate has been pre-coated with a monoclonal antibody specific for TAT. Standards or samples are then added to the microliter plate wells and TAT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TAT present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TAT are added to each well to "sandwich" the TAT immobilized on the plate. The microliter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TAT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TAT concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ADK70247.1
ncbi mol weight :
8,343 Da