ELISA/assay

Rat 3-Hydroxy-3-methylglutaryl CoA reductase ELISA Kit

MBS743245

48-Strip-Wells

470 USD

ELISA Kit

Rat 3-Hydroxy-3-methylglutaryl CoA reductase ELISA Kit

3-Hydroxy-3-methylglutaryl CoA reductase

3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMG-CoA reductase; 3-hydroxy-3-methylglutaryl-CoA reductase; 3-hydroxy-3-methylglutaryl-Coenzyme A reductase

HMGCR

Hmgcr; Hmgcr; Red; HMG-CoAR; HMG-CoA reductase

HMDH_MOUSE

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ug/mL

Intended Uses: This HMG-CoA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HMG-CoA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: HMG-CoA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HMG-CoA antibody and an HMG-CoA-HRP conjugate. The assay sample and buffer are incubated together with HMG-CoA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMG-CoA concentration since HMG-CoA from samples and HMG-CoA-HRP conjugate compete for the anti-HMG-CoA antibody binding site. Since the number of sites is limited, as more sites are occupied by HMG-CoA from the sample, fewer sites are left to bind HMG-CoA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMG-CoA concentration in each sample is interpolated from this standard curve.

160358778

NP_032281.2

NM_008255.2

97,040 Da

AMPK Signaling Pathway (989651); AMPK Signaling Pathway (992181); Activation Of Gene Expression By SREBF (SREBP) Pathway (1001054); Bile Secretion Pathway (193148); Bile Secretion Pathway (193095); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (421787); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (468288); Cholesterol Biosynthesis Pathway (198309); Cholesterol Biosynthesis Pathway (1001052); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (970709)

HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Protein type: EC 1.1.1.34; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis; Oxidoreductase. Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; integral to membrane. Molecular Function: protein homodimerization activity; hydroxymethylglutaryl-CoA reductase (NADPH) activity; protein phosphatase 2A binding; hydroxymethylglutaryl-CoA reductase activity; oxidoreductase activity; coenzyme binding; NADP binding; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor. Biological Process: steroid metabolic process; negative regulation of MAP kinase activity; cholesterol metabolic process; isoprenoid biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of skeletal muscle development; cholesterol biosynthetic process; positive regulation of stress-activated MAPK cascade; coenzyme A metabolic process; embryonic development; ubiquinone metabolic process; negative regulation of vasodilation; sterol biosynthetic process; positive regulation of cell proliferation; visual learning; lipid metabolic process; steroid biosynthetic process; protein tetramerization; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675