Monkey Cytochrome C ELISA Kit | MyBioSource MBS743215 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Cytochrome C ELISA Kit

catalog :

MBS743215

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS743215

products type :

ELISA Kit

products full name :

Monkey Cytochrome C ELISA Kit

products short name :

Cytochrome C

other names :

cytochrome c; Cytochrome c; cytochrome c; cytochrome c, somatic

products gene name :

Cy.C

other gene names :

CYCS; CYCS; CYC; HCS; THC4; CYC

uniprot entry name :

CYC_HUMAN

reactivity :

Monkey

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive

other info2 :

Intended Uses: This Cy.C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey Cy.C. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This Cy.C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey Cy.C. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cancer

products description :

Principle of the Assay: Cy.C ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti- Cy.C antibody and an Cy.C-HRP conjugate.The assay sample and buffer are incubated together with Cy.C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Cy.C concentration since Cy.C from samples and Cy.C-HRP conjugate compete for the anti-Cy.C antibody binding site. Since the number of sites is limited, as more sites are occupied by Cy.C from the sample, fewer sites are left to bind Cy.C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Cy.C concentration in each sample is interpolated from this standard curve.

ncbi gi num :

11128019

ncbi acc num :

NP_061820.1

ncbi gb acc num :

NM_018947.5

ncbi mol weight :

11,749 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (105672); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470)

ncbi summary :

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

uniprot summary :

CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family. Protein type: Oxidoreductase; Protein phosphatase, Ser/Thr (non-receptor); Apoptosis; Mitochondrial. Chromosomal Location of Human Ortholog: 7p15.3. Cellular Component: protein phosphatase type 2A complex; mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; nucleus; cytosol. Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; protein binding; iron ion binding; heme binding; protein serine/threonine phosphatase activity. Biological Process: mitochondrion organization and biogenesis; cellular metabolic process; response to reactive oxygen species; dephosphorylation; apoptosis; organelle organization and biogenesis; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; DNA fragmentation during apoptosis; caspase activation via cytochrome c; transmembrane transport; mitochondrial electron transport, ubiquinol to cytochrome c. Disease: Thrombocytopenia 4

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage