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ELISA/assay

Rat Syndecan-1/CD138 ELISA Kit

MBS743197

96 Strip Wells

675 USD

MBS743197

ELISA Kit

Rat Syndecan-1/CD138 ELISA Kit

Syndecan-1/CD138

syndecan-1; Syndecan-1; syndecan-1; CD138 antigen; syndecan proteoglycan 1; heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; CD_antigen: CD138

SDC1/CD138

SDC1; SDC1; SDC; CD138; SYND1; syndecan; SDC; SYND1

SDC1_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of SDC-1. No significant cross-reactivity or interference between SDC-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SDC-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This SDC-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SDC-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Cell Biology

Principle of the assay: SDC-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SDC-1. Standards or samples are then added to the microtiter plate wells and SDC-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SDC-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SDC-1 are added to each well to "sandwich" the SDC-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SDC-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDC-1 concentration in each sample is interpolated from this standard curve.

55749480

NP_001006947.1

NM_001006946.1

32,462 Da

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway 645305!!Arf6 Trafficking Events Pathway 137954!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway 645308!!Chylomicron-mediated Lipid Transport Pathway 106157!!Disease Pathway 530764!!Diseases Associated With Visual Transduction Pathway 771581!!ECM-receptor Interaction Pathway 83068!!ECM-receptor Interaction Pathway 479

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD