catalog number :
MBS743164
products type :
ELISA Kit
products full name :
Canine Troponin I ELISA Kit
products short name :
Troponin I
other names :
troponin I; troponin I; N/A
products gene name :
Tn- I
other gene names :
LOC100136571; N/A
specificity :
This assay has high sensitivity and excellent specificity for detection of cTnI. No significant cross-reactivity or interference between cTnI and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between cTnI and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This cTnI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine cTnI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
other info2 :
Sensitivity: The sensitivity in this assay is 0.1 ng/mL.
products categories :
Signal Transduction
products description :
Principle of the assay: cTnI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-cTnI antibody and an cTnI-HRP conjugate. The assay sample and buffer are incubated together with cTnI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cTnI concentration since cTnI from samples and cTnI-HRP conjugate compete for the anti-cTnI antibody binding site. Since the number of sites is limited, as more sites are occupied by cTnI from the sample, fewer sites are left to bind cTnI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cTnI concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001117133.1
ncbi gb acc num :
NM_001123661.1