ELISA/assay

Mouse alpha Lactalbumin ELISA Kit

MBS743094

48-Strip-Wells

470 USD

ELISA Kit

Mouse alpha Lactalbumin ELISA Kit

alpha Lactalbumin

Mouse a Lactalbumin ELISA Kit

alpha-lactalbumin; Alpha-lactalbumin; alpha-lactalbumin; lysozyme-like protein 7; lactose synthase B protein; lactalbumin, alpha-; Lactose synthase B protein; Lysozyme-like protein 7

alpha-La

a-La

LALBA; LALBA; LYZL7

LALBA_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This ?LALB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ?LALB. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY ?LALB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-?LALB antibody and an ?LALB-HRP conjugate. The assay sample and buffer are incubated together with ?LALB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ?LALB concentration since ?LALB from samples and ?LALB-HRP conjugate compete for the anti-?LALB antibody binding site. Since the number of sites is limited, as more sites are occupied by ?LALB from the sample, fewer sites are left to bind ?LALB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ?LALB concentration in each sample is interpolated from this standard curve.

4504947

NP_002280.1

NM_002289.2

16,225 Da

Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292); Metabolic Pathways (132956)

This gene encodes alpha-lactalbumin, a principal protein of milk. Alpha-lactalbumin forms the regulatory subunit of the lactose synthase (LS) heterodimer and beta 1,4-galactosyltransferase (beta4Gal-T1) forms the catalytic component. Together, these proteins enable LS to produce lactose by transfering galactose moieties to glucose. As a monomer, alpha-lactalbumin strongly binds calcium and zinc ions and may possess bactericidal or antitumor activity. A folding variant of alpha-lactalbumin, called HAMLET, likely induces apoptosis in tumor and immature cells. [provided by RefSeq, Jul 2008]

LALBA: Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins. Belongs to the glycosyl hydrolase 22 family. Protein type: Transferase; Apoptosis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: extracellular space. Molecular Function: lactose synthase activity; calcium ion binding. Biological Process: cell-cell signaling; apoptosis; defense response to bacterium; signal transduction; lactose biosynthetic process

48-Strip-Wells

470 USD

96-Strip-Wells

675