ELISA/assay

Chicken Toll Like Receptor4 ELISA Kit

MBS743084

48-Strip-Wells

440 USD

ELISA Kit

Chicken Toll Like Receptor4 ELISA Kit

[Toll Like Receptor4]

[TLR4]

[TLR4]

Chicken

This assay has high sensitivity and excellent specificity for detection of TLR-4. No significant cross-reactivity or interference between TLR-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TLR-4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Immunology

Intended Uses: This TLR-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken TLR-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TLR-4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TLR-4 antibody and an TLR-4-HRP conjugate. The assay sample and buffer are incubated together with TLR-4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLR-4 concentration since TLR-4 from samples and TLR-4-HRP conjugate compete for the anti-TLR-4 antibody binding site. Since the number of sites is limited, as more sites are occupied by TLR-4 from the sample, fewer sites are left to bind TLR-4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR-4 concentration in each sample is interpolated from this standard curve.

260401829

ACX37356.1

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415