Monkey Lipolysaccharide binding protein ELISA Kit | MyBioSource MBS742909 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Lipolysaccharide binding protein ELISA Kit

catalog :

MBS742909

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS742909

products type :

ELISA Kit

products full name :

Monkey Lipolysaccharide binding protein ELISA Kit

products short name :

Lipolysaccharide binding protein

other names :

lipopolysaccharide-binding protein; Lipopolysaccharide-binding protein; lipopolysaccharide-binding protein; LPS-binding protein; BPI fold containing family D, member 2; lipopolysaccharide binding protein

products gene name :

LBP

other gene names :

LBP; LBP; BPIFD2; LBP

uniprot entry name :

LBP_HUMAN

reactivity :

Monkey

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

products categories :

Immunology

products description :

Intended Uses: This LBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey LBP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: LBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LBP antibody and an LBP-HRP conjugate. The assay sample and buffer are incubated together with LBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LBP concentration since LBP from samples and LBP-HRP conjugate compete for the anti-LBP antibody binding site. Since the number of sites is limited, as more sites are occupied by LBP from the sample, fewer sites are left to bind LBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LBP concentration in each sample is interpolated from this standard curve.

ncbi gi num :

31652249

ncbi acc num :

NP_004130.2

ncbi gb acc num :

NM_004139.4

ncbi mol weight :

53,384 Da

ncbi pathways :

IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Innate Immune System Pathway (106387); NF-kappa B Signaling Pathway (634527); Regulation Of Toll-like Receptor Signaling Pathway (920955); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149); Toll Like Receptor 4 (TLR4) Cascade Pathway (106398); Toll-Like Receptors Cascades Pathway (106388); Toll-like Receptor Signaling Pathway (83076)

ncbi summary :

The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]

uniprot summary :

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q11.23. Cellular Component: extracellular space; cell surface; extracellular region. Molecular Function: protein binding; lipopolysaccharide binding; receptor binding. Biological Process: positive regulation of tumor necrosis factor biosynthetic process; detection of molecule of bacterial origin; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of interleukin-6 production; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; defense response to Gram-negative bacterium; positive regulation of tumor necrosis factor production; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production; positive regulation of interleukin-8 production; lipopolysaccharide transport; defense response to Gram-positive bacterium; macrophage activation during immune response; lipopolysaccharide-mediated signaling pathway; acute-phase response; toll-like receptor signaling pathway; cellular defense response; innate immune response; opsonization; toll-like receptor 4 signaling pathway; positive regulation of macrophage activation

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage