ELISA/assay

Monkey Insulin-like growth factor-2 ELISA Kit

MBS742743

48-Strip-Wells

470 USD

ELISA Kit

Monkey Insulin-like growth factor-2 ELISA Kit

Insulin-like growth factor-2

insulin-like growth factor II; Insulin-like growth factor II; insulin-like growth factor II; insulin-like growth factor type 2; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor 2; Somatomedin-ACleaved into the following 3 chains:Insulin-like growth factor II; Insulin-like growth factor II Ala-25 Del; Preptin

IGF-2

IGF2; IGF2; IGF-II; PP9974; C11orf43; PP1446; IGF-II

IGF2_HUMAN

Monkey

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses: This IGF-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IGF-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the Assay: IGF-2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IGF-2. Standards or samples are then added to the microtiter plate wells and IGF-2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IGF-2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for IGF-2 are added to each well to "sandwich" the IGF-2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IGF-2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF-2 concentration in each sample is interpolated from this standard curve.

189955

AAA60088.1

26,331 Da

Apoptosis Pathway (198797); Cardiac Progenitor Differentiation Pathway (712094); Endochondral Ossification Pathway (198812); Hedgehog Signaling Pathway (198835); Hemostasis Pathway (106028); IGF1R Signaling Cascade Pathway (730346); IRS-related Events Triggered By IGF1R Pathway (730348); Metabolism Of Proteins Pathway (106230); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050)

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

IGF2: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS). A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; extracellular region; plasma membrane. Molecular Function: insulin-like growth factor receptor binding; protein serine/threonine kinase activator activity; protein binding; growth factor activity; hormone activity; receptor activator activity; insulin receptor binding. Biological Process: positive regulation of catalytic activity; response to nicotine; genetic imprinting; multicellular organismal development; positive regulation of glycogen biosynthetic process; exocrine pancreas development; female pregnancy; positive regulation of activated T cell proliferation; response to estradiol stimulus; response to radiation; platelet degranulation; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; skeletal development; response to drug; response to nutrient levels; platelet activation; positive regulation of mitosis; glucose metabolic process; memory; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; organ morphogenesis; cellular protein metabolic process; response to ethanol; insulin receptor signaling pathway; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of insulin receptor signaling pathway; blood coagulation. Disease: Silver-russell Syndrome; Growth Restriction, Severe, With Distinctive Facies; Beckwith-wiedemann Syndrome; Wilms Tumor 1

48-Strip-Wells

470 USD

96-Strip-Wells

675