catalog number :
MBS742701
products type :
ELISA Kit
products full name :
Canine Adrenomedullin ELISA Kit
products short name :
Adrenomedullin
other names :
amd; Amd; Amd
other gene names :
amd; Dperamd; Dper_GL21130; GL21130
uniprot entry name :
B4GXD8_DROPE
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This AMD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine AMD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Neurobiology
products description :
Principle of the Assay: AMD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AMD antibody and an AMD-HRP conjugate. The assay sample and buffer are incubated together with AMD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMD concentration since AMD from samples and AMD-HRP conjugate compete for the anti-AMD antibody binding site. Since the number of sites is limited, as more sites are occupied by AMD from the sample, fewer sites are left to bind AMD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMD concentration in each sample is interpolated from this standard curve.
ncbi acc num :
EDW27249.1
ncbi mol weight :
47,692 Da