Monkey Apolipoprotein C1 ELISA Kit | MyBioSource MBS742590 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Apolipoprotein C1 ELISA Kit

catalog :

MBS742590

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS742590

products type :

ELISA Kit

products full name :

Monkey Apolipoprotein C1 ELISA Kit

products short name :

Apolipoprotein C1

other names :

apolipoprotein C-I; Apolipoprotein C-I; apolipoprotein C-I; apo-CIB; apoC-IB; apolipoprotein C1; apolipoprotein C-I variant I; apolipoprotein C-I; Apolipoprotein C1Cleaved into the following chain:Truncated apolipoprotein C-I

products gene name :

APOC1

other gene names :

APOC1; APOC1

uniprot entry name :

APOC1_HUMAN

reactivity :

Monkey

specificity :

This assay has high sensitivity and excellent specificity for detection of ApoC1. No significant cross-reactivity or interference between ApoC1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ApoC1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

other info2 :

Intended Uses: This ApoC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ApoC1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cardiovascular

products description :

Principle of the Assay: ApoC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ApoC1 antibody and an ApoC1-HRP conjugate. The assay sample and buffer are incubated together with ApoC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoC1 concentration since ApoC1 from samples and ApoC1-HRP conjugate compete for the anti-ApoC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoC1 from the sample, fewer sites are left to bind ApoC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoC1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4502157

ncbi acc num :

NP_001636.1

ncbi gb acc num :

NM_001645.3

ncbi mol weight :

9,332 Da

ncbi pathways :

Statin Pathway (198852)

ncbi summary :

The protein encoded by this gene is a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

APOC1: Appears to modulate the interaction of APOE with beta- migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification. Belongs to the apolipoprotein C1 family. Protein type: Secreted, signal peptide; Inhibitor; Lipid-binding; Endoplasmic reticulum; Secreted. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: chylomicron; endoplasmic reticulum. Molecular Function: phospholipase inhibitor activity; lipase inhibitor activity; phosphatidylcholine binding; fatty acid binding. Biological Process: positive regulation of catalytic activity; cholesterol metabolic process; triacylglycerol metabolic process; phospholipid efflux; negative regulation of lipid catabolic process; negative regulation of cholesterol transport; lipoprotein metabolic process; negative regulation of receptor-mediated endocytosis; cholesterol efflux; negative regulation of lipoprotein lipase activity; lipid metabolic process; regulation of cholesterol transport; negative regulation of lipid metabolic process; negative regulation of fatty acid biosynthetic process

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage