ELISA/assay

Canine Interleukin 17 ELISA Kit

MBS742559

48-Strip-Wells

470 USD

ELISA Kit

Canine Interleukin 17 ELISA Kit

Interleukin 17

interleukin-17, partial; Interleukin-17; Interleukin-17

IL17

IL17

Q5QEX9_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of IL-17. No significant cross-reactivity or interference between IL-17 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-17 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This IL-17 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IL-17. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: IL-17 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-17 antibody and an IL-17-HRP conjugate. The assay sample and buffer are incubated together with IL-17-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-17 concentration since IL-17 from samples and IL-17-HRP conjugate compete for the anti-IL-17 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-17 from the sample, fewer sites are left to bind IL-17-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-17 concentration in each sample is interpolated from this standard curve.

55139064

AAV41220.1

933 Da

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

48-Strip-Wells

470 USD

96-Strip-Wells

675