ELISA/assay

Monkey Platelet Activating Factor Acetylhydrolase ELISA Kit

MBS742391

48-Strip-Wells

470 USD

ELISA Kit

Monkey Platelet Activating Factor Acetylhydrolase ELISA Kit

Platelet Activating Factor Acetylhydrolase

platelet-activating factor acetylhydrolase; platelet-activating factor acetylhydrolase; group VII phospholipase A2; lipoprotein-associated phospholipase A2

PAF-AH

PLA2G7; PAF-AH; Lp-PLA2

Monkey

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses: This PAF-AH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey PAF-AH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: PAF-AH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PAF-AH. Standards or samples are then added to the microtiter plate wells and PAF-AH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PAF-AH present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PAF-AH are added to each well to "sandwich" the PAF-AH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PAF-AH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAF-AH concentration in each sample is interpolated from this standard curve.

163915175

NP_001106484.1

NM_001113013.1

Ether Lipid Metabolism Pathway (84416); Ether Lipid Metabolism Pathway (365); Metabolic Pathways (132936); Metabolism Of Proteins Pathway (1014364); Peptide Hormone Metabolism Pathway (1014412); Synthesis, Secretion, And Deacylation Of Ghrelin Pathway (1014414)

48-Strip-Wells

470 USD

96-Strip-Wells

675