catalog number :
MBS742190
products type :
ELISA Kit
products full name :
Sheep Interferon gamma antibody ELISA Kit
products short name :
Interferon gamma antibody
products name syn :
Sheep Interferon g antibody ELISA Kit
other names :
interferon gamma; Interferon gamma; interferon gamma; IFN-gamma; immune interferon; Immune interferon
products gene name :
gamma IFN-Ab
products gene name syn :
g IFN-Ab
other gene names :
IFNG; IFNG; IFN-gamma
uniprot entry name :
IFNG_PANTR
specificity :
This assay has high sensitivity and excellent specificity for detection of IFN?Ab. No significant cross-reactivity or interference between IFN?Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFN?Ab and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This IFN?Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep IFN?Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the assay: IFN?Ab ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with? Interferon antigen. Standards or samples are then added to the microtiter plate wells and IFN?Ab if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of IFN?Ab present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated ? Interferon antigen is added to each well to “sandwich” the IFN?Ab immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFN?Ab and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFN?Ab concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001180594.1
ncbi gb acc num :
NM_001193665.1
ncbi mol weight :
16,804 Da
ncbi pathways :
African Trypanosomiasis Pathway (194375); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83835); Allograft Rejection Pathway (535); Amoebiasis Pathway (167309); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83790); Antigen Processing And Presentation Pathway (485); Chagas Disease (American Trypanosomiasis) Pathway (147805); Chagas Disease (American Trypanosomiasis) Pathway (147795)