ELISA/assay

Goat A Proliferation inducing ligand ELISA Kit

MBS742189

96 Strip Wells

675 USD

ELISA Kit

Goat A Proliferation inducing ligand ELISA Kit

A Proliferation inducing ligand

APRIL; Tumor necrosis factor ligand superfamily member 13; tumor necrosis factor ligand superfamily member 13; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2; tumor necrosis factor (ligand) superfamily, member 13; A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; CD_antigen: CD256

APRIL

TNFSF13; TNFSF13; APRIL; CD256; TALL2; ZTNF2; TALL-2; TRDL-1; UNQ383/PRO715; APRIL; TALL2; ZTNF2; UNQ383/PRO715; APRIL; TALL-2; TRDL-1

TNF13_HUMAN

Goat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This APRIL/CD256 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat APRIL/CD256. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Immunology

Principle of the Assay: APRIL/CD256 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for APRIL/CD256. Standards or samples are then added to the microtiter plate wells and APRIL/CD256 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of APRIL/CD256 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for APRIL/CD256 are added to each well to "sandwich" the APRIL/CD256 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain APRIL/CD256 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APRIL/CD256 concentration in each sample is interpolated from this standard curve.

24934972

AAL90442.1

36,589 Da

Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Gene Expression Pathway 105937!!HuR Stabilizes MRNA Pathway 187216!!Intestinal Immune Network For IgA Production Pathway 128760!!Intestinal Immune Network For IgA Production Pathway 128670!!Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway 187211!!Rheumatoid Arthritis Pathway 200309!!Rheumatoid Arthritis Pathway 200269!!Spinal Cord Injury Pathway 739007

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

96 Strip Wells

675 USD