catalog number :
MBS742180
products type :
ELISA Kit
products full name :
Sheep Melatonin ELISA Kit
products short name :
Melatonin
specificity :
This assay has high sensitivity and excellent specificity for detection of MLT. No significant cross-reactivity or interference between MLT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MLT and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
products categories :
Neurobiology
products description :
Intended Uses: This MLT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep MLT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay MLT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MLT antibody and an MLT-HRP conjugate. The assay sample and buffer are incubated together with MLT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MLT concentration since MLT from samples and MLT-HRP conjugate compete for the anti-MLT antibody binding site. Since the number of sites is limited, as more sites are occupied by MLT from the sample, fewer sites are left to bind MLT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MLT concentration in each sample is interpolated from this standard curve.