ELISA/assay

Porcine FMS Like Tyrosine Kinase 3 Ligand ELISA Kit

MBS742129

48-Strip-Wells

470 USD

ELISA Kit

Porcine FMS Like Tyrosine Kinase 3 Ligand ELISA Kit

FMS Like Tyrosine Kinase 3 Ligand

fms-related tyrosine kinase 3 ligand; fms-related tyrosine kinase 3 ligand; fms-like tyrosine kinase 3 ligand; fms-related tyrosine kinase 3 ligand<

Flt3L

FLT3LG

Porcine

This assay has high sensitivity and excellent specificity for detection of FmsLTK3. No significant cross-reactivity or interference between FmsLTK3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FmsLTK3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This FmsLTK3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine FmsLTK3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: FmsLTK3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FmsLTK3. Standards or samples are then added to the microtiter plate wells and FmsLTK3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FmsLTK3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FmsLTK3 are added to each well to "sandwich" the FmsLTK3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FmsLTK3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FmsLTK3 concentration in each sample is interpolated from this standard curve.

30794350

NP_851373.1

NM_181030.2

Cytokine-cytokine Receptor Interaction Pathway (84290); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (84317); Hematopoietic Cell Lineage Pathway (489); Pathways In Cancer (84340)

48-Strip-Wells

470 USD

96-Strip-Wells

675