ELISA/assay

Human 3 Hydroxy 3 methylglutaryl CoA reductase ELISA Kit

MBS742031

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human 3 Hydroxy 3 methylglutaryl CoA reductase ELISA Kit

3 Hydroxy 3 methylglutaryl CoA reductase

3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMG-CoA reductase; 3-hydroxy-3-methylglutaryl-CoA reductase; 3-hydroxy-3-methylglutaryl-Coenzyme A reductase

HMGCR

Hmgcr; Hmgcr; Red; HMG-CoAR; HMG-CoA reductase

HMDH_MOUSE

Human

This assay has high sensitivity and excellent specificity for detection of HMGCR. No significant cross-reactivity or interference between HMGCR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HMGCR and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Sensitivity: 0.1 ng/mL.

Cardiovascular

Intended Uses This HMGCR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HMGCR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HMGCR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HMGCR. Standards or samples are then added to the microtiter plate wells and HMGCR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HMGCR present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HMGCR are added to each well to "sandwich” the HMGCR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HMGCR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGCR concentration in each sample is interpolated from this standard curve.

160358778

NP_032281.2

NM_008255.2

97,040 Da

AMPK Signaling Pathway (989651); AMPK Signaling Pathway (992181); Activation Of Gene Expression By SREBF (SREBP) Pathway (1001054); Bile Secretion Pathway (193148); Bile Secretion Pathway (193095); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (421787); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (468288); Cholesterol Biosynthesis Pathway (198309); Cholesterol Biosynthesis Pathway (1001052); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (970709)

HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Protein type: Oxidoreductase; Motility/polarity/chemotaxis; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral; EC 1.1.1.34. Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; integral to membrane. Molecular Function: protein homodimerization activity; hydroxymethylglutaryl-CoA reductase (NADPH) activity; protein phosphatase 2A binding; hydroxymethylglutaryl-CoA reductase activity; oxidoreductase activity; coenzyme binding; NADP binding; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor. Biological Process: steroid metabolic process; cholesterol metabolic process; negative regulation of MAP kinase activity; isoprenoid biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of skeletal muscle development; cholesterol biosynthetic process; positive regulation of stress-activated MAPK cascade; coenzyme A metabolic process; embryonic development; ubiquinone metabolic process; negative regulation of vasodilation; sterol biosynthetic process; positive regulation of cell proliferation; visual learning; lipid metabolic process; steroid biosynthetic process; protein tetramerization; negative regulation of apoptosis

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675