ELISA/assay

Bovine Stromal Cell Derived Factor 1 ELISA Kit

MBS741957

48-Strip-Wells

470 USD

ELISA Kit

Bovine Stromal Cell Derived Factor 1 ELISA Kit

Stromal Cell Derived Factor 1

stromal cell-derived factor 1; stromal cell-derived factor 1; chemokine ligand 12b; stromal cell derived factor 1; stromal cell-derived factor-1 beta; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); chemokine (C-X-C motif) ligand 12

SDF1

CXCL12; SDF1

Bovine

This assay has high sensitivity and excellent specificity for detection of SDF-1. No significant cross-reactivity or interference between SDF-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SDF-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Immunology

Intended Uses: This SDF-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine SDF-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay SDF-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SDF-1 antibody and an SDF-1-HRP conjugate. The assay sample and buffer are incubated together with SDF-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SDF-1 concentration since SDF-1 from samples and SDF-1-HRP conjugate compete for the anti-SDF-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SDF-1 from the sample, fewer sites are left to bind SDF-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF-1 concentration in each sample is interpolated from this standard curve.

45383151

NP_989841.1

NM_204510.1

Cytokine-cytokine Receptor Interaction Pathway (119307); Cytokine-cytokine Receptor Interaction Pathway (460); Intestinal Immune Network For IgA Production Pathway (128762); Intestinal Immune Network For IgA Production Pathway (128670)

48-Strip-Wells

470 USD

96-Strip-Wells

675