ELISA/assay

Sheep Mullerian Inhibiting Substance/Anti Mullerian Hormone ELISA Kit

MBS741819

48-Strip-Wells

470 USD

ELISA Kit

Sheep Mullerian Inhibiting Substance/Anti Mullerian Hormone ELISA Kit

Mullerian Inhibiting Substance/Anti Mullerian Hormone

muellerian-inhibiting factor; Muellerian-inhibiting factor; muellerian-inhibiting factor; anti-Muellerian hormone; Mullerian inhibiting factor; Mullerian inhibiting substance; muellerian-inhibiting substance; anti-Mullerian hormone; Anti-Muellerian hormone; AMH; Muellerian-inhibiting substance; MIS

MIS/AMH

AMH; AMH; MIF; MIS; MIF; AMH; MIS

MIS_HUMAN

Sheep

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses: This AMH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep AMH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: AMH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AMH. Standards or samples are then added to the microtiter plate wells and AMH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AMH present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for AMH are added to each well to "sandwich" the AMH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AMH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMH concentration in each sample is interpolated from this standard curve.

157266298

NP_000470.2

NM_000479.3

59,195 Da

ALK2 Signaling Events Pathway (137983); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); TGF-beta Signaling Pathway (83064); TGF-beta Signaling Pathway (475); CAMP Signaling Pathway (1017634); CAMP Signaling Pathway (1019520)

Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. [provided by RefSeq, Jul 2008]

AMH: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin. Defects in AMH are the cause of persistent Muellerian duct syndrome type 1 (PMDS1). PMDS1 is a form of male pseudohermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males. Belongs to the TGF-beta family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: extracellular space; cytoplasm; extracellular region. Molecular Function: growth factor activity; hormone activity; transforming growth factor beta receptor binding; receptor binding. Biological Process: response to drug; sex determination; Mullerian duct regression; cell-cell signaling; gonadal mesoderm development; preantral ovarian follicle growth; response to organic cyclic substance; sex differentiation; urogenital system development; activation of NF-kappaB transcription factor; aging. Disease: Persistent Mullerian Duct Syndrome, Types I And Ii

48-Strip-Wells

470 USD

96-Strip-Wells

675