ELISA/assay

Mouse 3-Hydroxy-3-methylglutaryl CoA reductase ELISA Kit

MBS741751

96 Strip Wells

675 USD

ELISA Kit

Mouse 3-Hydroxy-3-methylglutaryl CoA reductase ELISA Kit

3-Hydroxy-3-methylglutaryl CoA reductase

3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMG-CoA reductase; 3-hydroxy-3-methylglutaryl-CoA reductase; 3-hydroxy-3-methylglutaryl-Coenzyme A reductase

HMG-CoA

Hmgcr; Hmgcr; Red; HMG-CoAR; HMG-CoA reductase

HMDH_MOUSE

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This HMGCR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HMGCR. This ELISA kit for research use only, not for therapeutic or diagnostic applications

Sensitivity: The sensitivity in this assay is 0.1 ug/mL.

Cardiovascular

Principle of the Assay: HMGCR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HMGCR antibody and an HMGCR-HRP conjugate. The assay sample and buffer are incubated together with HMGCR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMGCR concentration since HMGCR from samples and HMGCR-HRP conjugate compete for the anti-HMGCR antibody binding site. Since the number of sites is limited, as more sites are occupied by HMGCR from the sample, fewer sites are left to bind HMGCR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGCR concentration in each sample is interpolated from this standard curve.

160358778

NP_032281.2

NM_008255.2

97,040 Da

AMPK Signaling Pathway 989651!!AMPK Signaling Pathway 992181!!Activation Of Gene Expression By SREBF (SREBP) Pathway 1001054!!Bile Secretion Pathway 193148!!Bile Secretion Pathway 193095!!C5 Isoprenoid Biosynthesis, Mevalonate Pathway 421787!!C5 Isoprenoid Biosynthesis, Mevalonate Pathway 468288!!Cholesterol Biosynthesis Pathway 198309!!Cholesterol Biosynthesis Pathway 1001052!!Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway 970709

96 Strip Wells

675 USD