ELISA/assay

Porcine CytoKeratin 18 ELISA Kit

MBS741736

48-Strip-Wells

470 USD

ELISA Kit

Porcine CytoKeratin 18 ELISA Kit

CytoKeratin 18

cytokeratin 18; Keratin, type I cytoskeletal 18; keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein; keratin 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

CK 18

KRT18; KRT18; K18; CYK18; CYK18; PIG46; CK-18; K18

K1C18_HUMAN

Porcine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CY-18 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CY-18. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the Assay: CK 18 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CK 18 antibody and an CK 18-HRP conjugate. The assay sample and buffer are incubated together with CK 18-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CK 18 concentration since CK 18 from samples and CK 18-HRP conjugate compete for the anti-CK 18 antibody binding site. Since the number of sites is limited, as more sites are occupied by CK 18 from the sample, fewer sites are left to bind CK 18-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CK 18 concentration in each sample is interpolated from this standard curve.

34037

CAA31375.1

P05783

48,058 Da

Caspase Cascade In Apoptosis Pathway (137974); EGFR1 Signaling Pathway (198782); Pathogenic Escherichia Coli Infection Pathway (83103); Pathogenic Escherichia Coli Infection Pathway (672459); Pathogenic Escherichia Coli Infection Pathway (516)

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis. Protein type: Cytoskeletal; Nucleolus. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: perinuclear region of cytoplasm; cytoplasm; keratin filament; nucleolus; intermediate filament; microtubule organizing center. Molecular Function: protein binding; structural molecule activity. Biological Process: anatomical structure morphogenesis; tumor necrosis factor-mediated signaling pathway; viral reproduction; intermediate filament cytoskeleton organization and biogenesis; cell cycle; negative regulation of apoptosis; Golgi to plasma membrane CFTR protein transport. Disease: Cirrhosis, Familial

48-Strip-Wells

470 USD

96-Strip-Wells

675