ELISA/assay

Monkey A Disintegrin And Metalloprotease 8 ELISA Kit

MBS741637

96 Strip Wells

675 USD

ELISA Kit

Monkey A Disintegrin And Metalloprotease 8 ELISA Kit

A Disintegrin And Metalloprotease 8

ADAM8 protein; Disintegrin and metalloproteinase domain-containing protein 8; disintegrin and metalloproteinase domain-containing protein 8; cell surface antigen MS2; human leukocyte differentiation antigen; a disintegrin and metalloproteinase domain 8; ADAM metallopeptidase domain 8; Cell surface antigen MS2; CD_antigen: CD156a

ADAM8

ADAM8; ADAM8; MS2; CD156; CD156a; MS2; ADAM 8

ADAM8_HUMAN

Monkey

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This ADAM8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ADAM8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This ADAM8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ADAM8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: ADAM8 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADAM8. Standards or samples are then added to the microtiter plate wells and ADAM8 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADAM8 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADAM8 are added to each well to "sandwich" the ADAM8 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADAM8 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM8 concentration in each sample is interpolated from this standard curve.

109731932

AAI15405.1

80,262 Da

Degradation Of The Extracellular Matrix Pathway 576263!!Extracellular Matrix Organization Pathway 576262

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]

96 Strip Wells

675 USD