product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Monkey alpha1 Antitrypsin ELISA Kit
catalog :
MBS741620
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS741620
products type :
ELISA Kit
products full name :
Monkey alpha1 Antitrypsin ELISA Kit
products short name :
alpha1 Antitrypsin
products name syn :
Monkey a1 Antitrypsin ELISA Kit
other names :
alpha1-antitrypsin; Alpha-1-antiproteinase; Alpha-1-antitrypsin; Alpha-1-proteinase inhibitor
products gene name :
alpha1-AT
products gene name syn :
a1-AT
uniprot entry name :
A1AT_CALCN
reactivity :
Monkey
specificity :
This assay has high sensitivity and excellent specificity for detection of alpha1-AT. No significant cross-reactivity or interference between alpha1-AT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between alpha1-AT and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
other info2 :
Intended Uses: This alpha1-AT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey alpha1-AT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cardiovascular
products description :
Principle of the assay: alpha1-AT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-alpha1-AT antibody and an alpha1-AT-HRP conjugate. The assay sample and buffer are incubated together with alpha1-AT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha1-AT concentration since alpha1-AT from samples and alpha1-AT-HRP conjugate compete for the anti-alpha1-AT antibody binding site. Since the number of sites is limited, as more sites are occupied by alpha1-AT from the sample, fewer sites are left to bind alpha1-AT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha1-AT concentration in each sample is interpolated from this standard curve.
ncbi gi num :
2804195
ncbi acc num :
BAA24422.1
ncbi mol weight :
45,729 Da
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!