ELISA/assay

Porcine A Disintegrin And Metalloprotease 8 ELISA Kit

MBS741606

96 Strip Wells

675 USD

ELISA Kit

Porcine A Disintegrin And Metalloprotease 8 ELISA Kit

A Disintegrin And Metalloprotease 8

ADAM8 protein; Disintegrin and metalloproteinase domain-containing protein 8; disintegrin and metalloproteinase domain-containing protein 8; cell surface antigen MS2; human leukocyte differentiation antigen; a disintegrin and metalloproteinase domain 8; ADAM metallopeptidase domain 8; Cell surface antigen MS2; CD_antigen: CD156a

ADAM8

ADAM8; ADAM8; MS2; CD156; CD156a; MS2; ADAM 8

ADAM8_HUMAN

Porcine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This ADAM8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ADAM8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Cardiovascular

Principle of the Assay: ADAM8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM8 antibody and an ADAM8-HRP conjugate. The assay sample and buffer are incubated together with ADAM8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM8 concentration since ADAM8 from samples and ADAM8-HRP conjugate compete for the anti-ADAM8 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM8 from the sample, fewer sites are left to bind ADAM8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM8 concentration in each sample is interpolated from this

109731932

AAI15405.1

80,262 Da

Degradation Of The Extracellular Matrix Pathway 576263!!Extracellular Matrix Organization Pathway 576262

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]

96 Strip Wells

675 USD