ELISA/assay

Chicken Interferon gamma ELISA Kit

MBS741374

48-Strip-Wells

440 USD

ELISA Kit

Chicken Interferon gamma ELISA Kit

[Interferon gamma]

[Chicken Interferon g ELISA Kit]

[interferon gamma; Interferon gamma; interferon gamma; IFN-gamma; immune interferon; interferon, gamma; Immune interferon]

[IFN-gamma]

[IFN-g]

[IFNG; IFNG; IFG; IFI; IFN-gamma]

IFNG_HUMAN

Chicken

This assay has high sensitivity and excellent specificity for detection of IFNbeta. No significant cross-reactivity or interference between IFNbeta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFNbeta and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

Cancer

Intended Uses: This IFNbeta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IFNbeta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IFNbeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IFNbeta antibody and an IFNbeta-HRP conjugate. The assay sample and buffer are incubated together with IFNbeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNbeta concentration since IFNbeta from samples and IFNbeta-HRP conjugate compete for the anti-IFNbeta antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNbeta from the sample, fewer sites are left to bind IFNbeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNbeta concentration in each sample is interpolated from this standard curve.

56786138

NP_000610.2

NM_000619.2

P01579

19,348 Da

ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993)

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family. Protein type: Secreted, signal peptide; Cytokine; Secreted; Membrane protein, integral. Chromosomal Location of Human Ortholog: 12q14. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: interferon-gamma receptor binding; cytokine activity. Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; positive regulation of interleukin-23 production; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-12 production; apoptosis; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; cytokine and chemokine mediated signaling pathway; response to virus; defense response to protozoan; positive regulation of tumor necrosis factor production; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of chemokine biosynthetic process; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion. Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415