ELISA/assay

Porcine Peptide YY ELISA Kit

MBS741355

48-Strip-Wells

470 USD

ELISA Kit

Porcine Peptide YY ELISA Kit

Peptide YY

PYY; Peptide YY; peptide YY; prepro-PYY; peptide tyrosine tyrosine; peptide YY; PYY-I; Peptide tyrosine tyrosineCleaved into the following chain:Peptide YY(3-36)Alternative name(s):PYY-II

PYY

PYY; PYY; PYY1; PYY-I; PYY

PYY_HUMAN

Porcine

This assay has high sensitivity and excellent specificity for detection of PYY. No significant cross-reactivity or interference between PYY and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PYY and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL. Assay Type: Competitive

Intended Uses: This PYY ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine PYY. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the Assay: PYY ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PYY antibody and an PYY-HRP conjugate. The assay sample and buffer are incubated together with PYY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PYY concentration since PYY from samples and PYY-HRP conjugate compete for the anti-PYY antibody binding site. Since the number of sites is limited, as more sites are occupied by PYY from the sample, fewer sites are left to bind PYY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PYY concentration in each sample is interpolated from this standard curve.

49457254

CAG46926.1

P10082

10,360 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

The protein encoded by this gene is proteolytically processed to release a peptide that inhibits pancreatic secretion and mobility in the gut. Rare variations in this gene could increase susceptibility to obesity. [provided by RefSeq, Jul 2010]

PYY: This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. Belongs to the NPY family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17q21.1. Cellular Component: extracellular space; extracellular region. Molecular Function: neuropeptide hormone activity; protein binding; G-protein-coupled receptor binding; hormone activity. Biological Process: G-protein coupled receptor protein signaling pathway; cell proliferation; cell-cell signaling; neuropeptide signaling pathway; eating behavior; regulation of appetite; digestion; feeding behavior; cytoskeleton organization and biogenesis; cell motility. Disease: Obesity

48-Strip-Wells

470 USD

96-Strip-Wells

675