Porcine Somatostatin ELISA Kit | MyBioSource MBS741330 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine Somatostatin ELISA Kit

catalog :

MBS741330

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS741330

products type :

ELISA Kit

products full name :

Porcine Somatostatin ELISA Kit

products short name :

[Somatostatin]

other names :

[Somatostatin; Somatostatin; somatostatin; somatostatin-14; somatostatin-28; prepro-somatostatin; growth hormone release-inhibiting factor; somatostatin; Growth hormone release-inhibiting factorCleaved into the following 2 chains:Somatostatin-28; Somatostatin-14]

products gene name :

[SST]

other gene names :

[SST; SST; SMST]

uniprot entry name :

SMS_HUMAN

reactivity :

Porcine

specificity :

This assay has high sensitivity and excellent specificity for detection of SST. No significant cross-reactivity or interference between SST and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SST and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Sandwich. Sensitivity: 1.0 pg/mL.

products categories :

Neurobiology

products description :

Intended Uses: This SST ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SST. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: SST ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SST. Standards or samples are then added to the microtiter plate wells and SST if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SST present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SST are added to each well to "sandwich" the SST immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SST and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SST concentration in each sample is interpolated from this standard curve.

ncbi gi num :

21619156

ncbi acc num :

AAH32625.1

ncbi mol weight :

12,736 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383); Peptide Ligand-binding Receptors Pathway (106358); SIDS Susceptibility Pathways (198901); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

ncbi summary :

The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. [provided by RefSeq, Jul 2008]

uniprot summary :

SST: Somatostatin inhibits the release of somatotropin. Belongs to the somatostatin family. Protein type: Secreted; Hormone; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q28. Cellular Component: extracellular space; cell soma; extracellular region. Molecular Function: hormone activity. Biological Process: response to drug; negative regulation of cell proliferation; synaptic transmission; G-protein coupled receptor protein signaling pathway; hyperosmotic response; cell surface receptor linked signal transduction; cell-cell signaling; induction of apoptosis by hormones; response to heat; response to steroid hormone stimulus; digestion; response to amino acid stimulus; regulation of cell migration; response to nutrient

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage