ELISA/assay

Porcine Interleukin 8 ELISA Kit

MBS741307

48-Strip-Wells

440 USD

ELISA Kit

Porcine Interleukin 8 ELISA Kit

[Interleukin 8]

[interleukin-8; Interleukin-8; interleukin-8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8]

[IL-8]

[CXCL8; CXCL8; IL8; IL8; IL-8]

IL8_MACMU

Porcine

101

This assay has high sensitivity and excellent specificity for detection of IL-8. No significant cross-reactivity or interference between IL-8 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-8 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

Immunology

Intended Uses: This IL-8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine IL-8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IL-8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-8 antibody and an IL-8-HRP conjugate. The assay sample and buffer are incubated together with IL-8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-8 concentration since IL-8 from samples and IL-8-HRP conjugate compete for the anti-IL-8 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-8 from the sample, fewer sites are left to bind IL-8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-8 concentration in each sample is interpolated from this standard curve.

74136483

NP_001028137.1

NM_001032965.1

P67813

11,320 Da

Amoebiasis Pathway (167336); Amoebiasis Pathway (167191); Bladder Cancer Pathway (86781); Bladder Cancer Pathway (527); Chagas Disease (American Trypanosomiasis) Pathway (147811); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Signaling Pathway (99278); Chemokine Signaling Pathway (96864); Cytokine-cytokine Receptor Interaction Pathway (86721); Cytokine-cytokine Receptor Interaction Pathway (460)

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415