ELISA/assay

Canine Chemokine interferon-inducible protein 10 ELISA Kit

MBS741234

48-Strip-Wells

470 USD

ELISA Kit

Canine Chemokine interferon-inducible protein 10 ELISA Kit

Chemokine interferon-inducible protein 10

IP-10; C-X-C motif chemokine 10; C-X-C motif chemokine 10; gamma-IP10; interferon activated gene 10; small-inducible cytokine B10; interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; 10 kDa interferon gamma-induced protein; small inducible cytokine B subfamily (Cys-X-Cys), member 10; chemokine (C-X-C motif) ligand 10; 10 kDa interferon gamma-induced protein; Gamma-IP10; IP-10; C7; Interferon-gamma induced protein CRG-2; Small-inducible cytokine B10

IP-10

Cxcl10; Cxcl10; C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10; Crg2; Ifi10; Inp10; Scyb10; Gamma-IP10; IP-10

CXL10_MOUSE

Canine

This assay has high sensitivity and excellent specificity for detection of IP-10. No significant cross-reactivity or interference between IP-10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IP-10 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This IP-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IP-10. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: IP-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IP-10 antibody and an IP-10-HRP conjugate. The assay sample and buffer are incubated together with IP-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IP-10 concentration since IP-10 from samples and IP-10-HRP conjugate compete for the anti-IP-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by IP-10 from the sample, fewer sites are left to bind IP-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IP-10 concentration in each sample is interpolated from this standard curve.

976159

AAA75249.1

10,789 Da

Chemokine Receptors Bind Chemokines Pathway (1000944); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125146); Cytosolic DNA-sensing Pathway (124833); G Alpha (i) Signalling Events Pathway (1000973)

CXCL10: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide; Secreted. Cellular Component: extracellular space; cell; extracellular region; external side of plasma membrane. Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity. Biological Process: positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; chemotaxis; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of cell proliferation; immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of myoblast differentiation; inflammatory response; defense response to virus; positive regulation of cell migration

48-Strip-Wells

470 USD

96-Strip-Wells

675