catalog number :
MBS741104
products type :
ELISA Kit
products full name :
Porcine Secretory Immunoglobulin A ELISA Kit
products short name :
Secretory Immunoglobulin A
products gene name :
SIgA
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ug/mL.
other info2 :
Intended Uses: This SIGA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine SIGA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the Assay: SIGA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SIGA antibody and an SIGA-HRP conjugate. The assay sample and buffer are incubated together with SIGA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SIGA concentration since SIGA from samples and SIGA-HRP conjugate compete for the anti-SIGA antibody binding site. Since the number of sites is limited, as more sites are occupied by SIGA from the sample, fewer sites are left to bind SIGA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIGA concentration in each sample is interpolated from this standard curve.