ELISA/assay

Monkey Dipeptidyl Peptidase IV ELISA Kit

MBS740987

48-Strip-Wells

440 USD

ELISA Kit

Monkey Dipeptidyl Peptidase IV ELISA Kit

[Dipeptidyl Peptidase IV]

[dipeptidyl peptidase 4; Dipeptidyl peptidase 4; dipeptidyl peptidase 4; ADCP-2; DPP IV; dipeptidylpeptidase 4; dipeptidyl peptidase IV; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); dipeptidyl-peptidase 4; ADABP; Adenosine deaminase complexing protein 2; ADCP-2; Dipeptidyl peptidase IV; DPP IV; T-cell activation antigen CD26; TP103; CD_antigen: CD26Cleaved into the following 2 chains:Dipeptidyl peptidase 4 membrane formAlternative name(s):Dipeptidyl peptidase IV membrane form]

[DPP4]

[DPP4; DPP4; CD26; ADABP; ADCP2; DPPIV; TP103; ADCP2; CD26; ADCP-2; DPP IV]

DPP4_HUMAN

Monkey

This assay has high sensitivity and excellent specificity for detection of DNase B Ab. No significant cross-reactivity or interference between DNase B Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DNase B Ab and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This DNase B Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DNase B Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: DNase B Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Deoxyribonuclease B antigen and an DNase B Ab-HRP conjugate. The assay sample and buffer are incubated together with DNase B Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DNase B Ab concentration since DNase B Ab from samples and DNase B Ab-HRP conjugate compete for the Deoxyribonuclease B antigen binding site. Since the number of sites is limited, as more sites are occupied by DNase B Ab from the sample, fewer sites are left to bind DNase B Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DNase B Ab concentration in each sample is interpolated from this standard curve.

18765694

NP_001926.2

NM_001935.3

88,279 Da

Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868); Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway (187171); Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway (187172)

The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]

DPP4: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF- kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Belongs to the peptidase S9B family. DPPIV subfamily. Protein type: Membrane protein, integral; Protease; EC 3.4.14.5; Cell surface; Cell adhesion. Chromosomal Location of Human Ortholog: 2q24.3. Cellular Component: intercellular canaliculus; cell surface; focal adhesion; membrane; endocytic vesicle; lysosomal membrane; lamellipodium; apical plasma membrane; integral to membrane; plasma membrane; lipid raft. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protease binding; serine-type peptidase activity; dipeptidyl-peptidase activity; serine-type endopeptidase activity; receptor binding. Biological Process: regulation of cell-cell adhesion mediated by integrin; T cell activation; positive regulation of cell proliferation; T cell costimulation; response to hypoxia; endothelial cell migration; proteolysis

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415