ELISA/assay

Monkey Hepatocyte Growth Factor ELISA Kit

MBS740982

48-Strip-Wells

470 USD

ELISA Kit

Monkey Hepatocyte Growth Factor ELISA Kit

Hepatocyte Growth Factor

hepatocyte growth factor isoform 3; Hepatocyte growth factor; hepatocyte growth factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoietin-A; Scatter factor; SF

HGF

HGF; HGF; SF; HGFB; HPTA; F-TCF; DFNB39; HPTA; SF

HGF_HUMAN

Monkey

This assay has high sensitivity and excellent specificity for detection of HGF. No significant cross-reactivity or interference between HGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HGF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses This HGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey HGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HGF antibody and an HGF-HRP conjugate. The assay sample and buffer are incubated together with HGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HGF concentration since HGF from samples and HGF-HRP conjugate compete for the anti-HGF antibody binding site. Since the number of sites is limited, as more sites are occupied by HGF from the sample, fewer sites are left to bind HGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HGF concentration in each sample is interpolated from this standard curve.

58533170

NP_001010932.1

NM_001010932.1

24,116 Da

Arf6 Signaling Events Pathway (138034); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Direct P53 Effectors Pathway (137939); FGF Signaling Pathway (137989); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Hemostasis Pathway (106028)

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

HGF: HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. Activating ligand for the receptor tyrosine kinase MET by binding and promoting its dimerization. Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39). A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the peptidase S1 family. Plasminogen subfamily. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Hormone; Motility/polarity/chemotaxis; Cell development/differentiation. Chromosomal Location of Human Ortholog: 7q21.1. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; serine-type endopeptidase activity; chemoattractant activity. Biological Process: positive regulation of myelination; platelet activation; mitosis; organ regeneration; activation of MAPK activity; myoblast proliferation; hepatocyte growth factor receptor signaling pathway; negative regulation of caspase activity; proteolysis; liver development; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of osteoblast differentiation; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; platelet degranulation; positive chemotaxis; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; hyaluronan metabolic process; positive regulation of cell migration. Disease: Deafness, Autosomal Recessive 39

48-Strip-Wells

470 USD

96-Strip-Wells

675