product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Canine Heat Shock Protein 70 ELISA Kit
catalog :
MBS740980
quantity :
48-Strip-Wells
price :
440 USD
more info or order :
image
image 1 :
product information
catalog number :
MBS740980
products type :
ELISA Kit
products full name :
Canine Heat Shock Protein 70 ELISA Kit
products short name :
[Heat Shock Protein 70]
other names :
[HSP70; HSP70; HSP70]
products gene name :
[HSP70]
uniprot entry name :
Q40058_HORVU
reactivity :
Canine
specificity :
This assay has high sensitivity and excellent specificity for detection of HSP-70. No significant cross-reactivity or interference between HSP-70 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP-70 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Detection Range: 0.5-10ng/mL?. Sensitivity: 0.1ng/mL
products categories :
Signal Transduction
products description :
Intended Uses: This HSP-70 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine HSP-70. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HSP-70 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSP-70 antibody and an HSP-70-HRP conjugate. The assay sample and buffer are incubated together with HSP-70-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP-70 concentration since HSP-70 from samples and HSP-70-HRP conjugate compete for the anti-HSP-70 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP-70 from the sample, fewer sites are left to bind HSP-70-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP-70 concentration in each sample is interpolated from this standard curve.
ncbi gi num :
476003
ncbi acc num :
AAA62325.1
ncbi mol weight :
67,016 Da
size1 :
48-Strip-Wells
price1 :
440 USD
size2 :
96-Strip-Wells
price2 :
640
size3 :
5x96-Strip-Wells
price3 :
2895
size4 :
10x96-Strip-Wells
price4 :
5415
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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