catalog number :
MBS740967
products type :
ELISA Kit
products full name :
Porcine Heat Shock Protein 90 ELISA Kit
products short name :
Heat Shock Protein 90
other names :
Heat shock protein 90; Heat shock protein 90; molecular chaperone and allergen Mod-E/Hsp90/Hsp1; 65 kDa IgE-binding protein; Heat shock protein hsp1; Allergen: Asp f 12
products gene name :
HSP-90
other gene names :
AFUA_5G04170; hsp90; hsp1; AFUA_5G04170
uniprot entry name :
HSP90_ASPFU
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
products categories :
Signal Transduction
products description :
Intended Uses: This HSP-90 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine HSP-90. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HSP-90 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSP-90 antibody and an HSP-90-HRP conjugate. The assay sample and buffer are incubated together with HSP-90-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP-90 concentration since HSP-90 from samples and HSP-90-HRP conjugate compete for the anti-HSP-90 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP-90 from the sample, fewer sites are left to bind HSP-90-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP-90 concentration in each sample is interpolated from this standard curve.
ncbi mol weight :
80,640 Da
ncbi pathways :
Protein Processing In Endoplasmic Reticulum Pathway (167357); Protein Processing In Endoplasmic Reticulum Pathway (167190)