catalog number :
MBS740884
products type :
ELISA Kit
products full name :
Canine Kidney Injury Molecule 1 ELISA Kit
products short name :
Kidney Injury Molecule 1
other names :
kidney injury molecule 1; Kidney injury molecule 1; Kidney injury molecule 1; Uncharacterized protein; Zgc:92647
products gene name :
KIM1
other gene names :
havcr1; kim1; zgc:92647
uniprot entry name :
Q6DH50_DANRE
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
products categories :
Immunology
products description :
Intended Uses: This KIM1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine KIM1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: KIM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KIM1 antibody and an KIM1-HRP conjugate. The assay sample and buffer are incubated together with KIM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KIM1 concentration since KIM1 from samples and KIM1-HRP conjugate compete for the anti-KIM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by KIM1 from the sample, fewer sites are left to bind KIM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KIM1 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ABP97434.1
ncbi mol weight :
29,575 Da