product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Bovine Endothelial Nitric Oxide Synthase ELISA Kit
catalog :
MBS740781
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS740781
products type :
ELISA Kit
products full name :
Bovine Endothelial Nitric Oxide Synthase ELISA Kit
products short name :
Endothelial Nitric Oxide Synthase
other names :
eNOS protein, partial; ENOS protein; ENOS protein
products gene name :
eNOS
other gene names :
eNOS
uniprot entry name :
A6H544_CALJA
reactivity :
Bovine
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive
other info2 :
Intended Uses: This eNOS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine eNOS. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This eNOS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine eNOS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cardiovascular
products description :
Principle of the Assay: eNOS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-eNOS antibody and an eNOS-HRP conjugate. The assay sample and buffer are incubated together with eNOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the eNOS concentration since eNOS from samples and eNOS-HRP conjugate compete for the anti-eNOS antibody binding site. Since the number of sites is limited, as more sites are occupied by eNOS from the sample, fewer sites are left to bind eNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The eNOS concentration in each sample is interpolated from this standard curve.
ncbi gi num :
148913721
ncbi acc num :
CAN84512.1
uniprot acc num :
A6H544
ncbi mol weight :
5,517 Da
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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