ELISA/assay

Monkey Transferrin ELISA Kit

MBS740730

48-Strip-Wells

470 USD

ELISA Kit

Monkey Transferrin ELISA Kit

Transferrin

transferrin; Serotransferrin; serotransferrin; siderophilin; beta-1 metal-binding globulin; transferrin; Beta-1 metal-binding globulin; Siderophilin

TF

TF; TF; TFQTL1; PRO1557; PRO2086; PRO1400; Transferrin

TRFE_HUMAN

Monkey

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 ng/mL.

Cardiovascular

Intended Uses: This TRF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey TRF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TRF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TRF. Standards or samples are then added to the microtiter plate wells and TRF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TRF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TRF are added to each well to "sandwich" the TRF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TRF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRF concentration in each sample is interpolated from this standard curve.

248648

AAB22049.1

77,064 Da

EPHB Forward Signaling Pathway (138047); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Hemostasis Pathway (106028); Iron Metabolism In Placenta Pathway (672461); Iron Uptake And Transport Pathway (187191); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050)

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3q22.1. Cellular Component: extracellular space; cell surface; cytoplasmic membrane-bound vesicle; early endosome; extracellular region; coated pit; recycling endosome; endocytic vesicle; perinuclear region of cytoplasm; apical plasma membrane; late endosome; basal plasma membrane; endosome membrane; vesicle. Molecular Function: protein binding; ferric iron binding; ubiquitin protein ligase binding. Biological Process: retinal homeostasis; platelet activation; platelet degranulation; cellular iron ion homeostasis; transferrin transport; blood coagulation; transmembrane transport. Disease: Atransferrinemia

48-Strip-Wells

470 USD

96-Strip-Wells

675