ELISA/assay

Chicken Matrix metalloproteinase 10 ELISA Kit

MBS740643

48-Strip-Wells

470 USD

ELISA Kit

Chicken Matrix metalloproteinase 10 ELISA Kit

Matrix metalloproteinase 10

matrix metalloproteinase 10 (stromelysin 2); Stromelysin-2; stromelysin-2; MMP-10; transin 2; transin-2; stromelysin 2; matrix metalloprotease 10; matrix metalloproteinase-10; matrix metalloproteinase 10 (stromelysin 2); matrix metallopeptidase 10 (stromelysin 2); Matrix metalloproteinase-10; MMP-10; Transin-2

MMP-10

MMP10; MMP10; SL-2; STMY2; STMY2; SL-2; MMP-10

MMP10_HUMAN

Chicken

This assay has high sensitivity and excellent specificity for detection of MMP-10. No significant cross-reactivity or interference between MMP-10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP-10 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This MMP-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken MMP-10. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the assay: MMP-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMP-10 antibody and an MMP-10-HRP conjugate. The assay sample and buffer are incubated together with MMP-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-10 concentration since MMP-10 from samples and MMP-10-HRP conjugate compete for the anti-MMP-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-10 from the sample, fewer sites are left to bind MMP-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-10 concentration in each sample is interpolated from this standard curve.

51988876

AAU21039.1

P09238

54,151 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900)

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

MMP10: Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase. Belongs to the peptidase M10A family. Protein type: EC 3.4.24.22; Secreted, signal peptide; Extracellular matrix; Secreted; Protease. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: zinc ion binding; metalloendopeptidase activity; calcium ion binding. Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis; regulation of cell migration

48-Strip-Wells

470 USD

96-Strip-Wells

675