ELISA/assay

Mouse A Disintegrin And Metalloprotease ELISA Kit

MBS740613

96 Strip Wells

675 USD

ELISA Kit

Mouse A Disintegrin And Metalloprotease ELISA Kit

A Disintegrin And Metalloprotease

A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin and metalloproteinase with thrombospondin motifs 7; COMPase; ADAMTS-7; ADAM-TS 7; metalloprotease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 7; a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 7; COMPase

ADAM

Adamts7; Adamts7; ADAM-TS7; ADAMTS7B; ADAM-TS 7; ADAM-TS7; ADAMTS-7

ATS7_MOUSE

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This ADAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ADAM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Cardiovascular

Principle of the Assay: ADAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM antibody and an ADAM-HRP conjugate. The assay sample and buffer are incubated together with ADAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM concentration since ADAM from samples and ADAM-HRP conjugate compete for the anti-ADAM antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM from the sample, fewer sites are left to bind ADAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM concentration in each sample is interpolated from this standard curve.

133778949

NP_001003911.2

NM_001003911.2

177,712 Da

Metabolism Of Proteins Pathway 970583!!O-glycosylation Of TSR Domain-containing Proteins Pathway 1000404!!O-linked Glycosylation Pathway 1000403!!Post-translational Protein Modification Pathway 1000373

96 Strip Wells

675 USD