Porcine Eosinophil chemotactic factor ELISA Kit | MyBioSource MBS740576 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine Eosinophil chemotactic factor ELISA Kit

catalog :

MBS740576

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS740576

products type :

ELISA Kit

products full name :

Porcine Eosinophil chemotactic factor ELISA Kit

products short name :

Eosinophil chemotactic factor

other names :

eotaxin; Eotaxin; eotaxin; eotaxin-1; eosinophil chemotactic protein; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin); chemokine (C-C motif) ligand 11; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11

products gene name :

ECF

other gene names :

CCL11; CCL11; SCYA11; SCYA11

uniprot entry name :

CCL11_HUMAN

reactivity :

Porcine

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

products categories :

Immunology

products description :

Intended Uses: This ECF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ECF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ECF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ECF antibody and an ECF-HRP conjugate. The assay sample and buffer are incubated together with ECF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ECF concentration since ECF from samples and ECF-HRP conjugate compete for the anti-ECF antibody binding site. Since the number of sites is limited, as more sites are occupied by ECF from the sample, fewer sites are left to bind ECF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ECF concentration in each sample is interpolated from this standard curve.

ncbi gi num :

2462478

ncbi acc num :

CAB07027.1

uniprot acc num :

P51671

ncbi mol weight :

10,732 Da

ncbi pathways :

Asthma Pathway (83120); Asthma Pathway (532); CXCR3-mediated Signaling Events Pathway (138011); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); GPCR Ligand Binding Pathway (161020)

ncbi summary :

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil-specific chemokine is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. [provided by RefSeq, Sep 2014]

uniprot summary :

CCL11: In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family. Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; chemokine activity. Biological Process: chronic inflammatory response; mast cell chemotaxis; response to virus; actin filament organization; cytoskeleton organization and biogenesis; chemotaxis; signal transduction; protein amino acid phosphorylation; cellular calcium ion homeostasis; regulation of cell shape; positive regulation of angiogenesis; response to radiation; positive regulation of actin filament polymerization; eosinophil chemotaxis; positive regulation of endothelial cell proliferation; immune response; cell adhesion; inflammatory response; positive regulation of cell migration. Disease: Asthma, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage