Monkey Insulin Like Growth Factor Binding Protein 2 ELISA Kit | MyBioSource MBS740575 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Insulin Like Growth Factor Binding Protein 2 ELISA Kit

catalog :

MBS740575

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS740575

products type :

ELISA Kit

products full name :

Monkey Insulin Like Growth Factor Binding Protein 2 ELISA Kit

products short name :

Insulin Like Growth Factor Binding Protein 2

other names :

Insulin-like growth factor-binding protein 2; Insulin-like growth factor-binding protein 2; insulin-like growth factor-binding protein 2; IBP-2; IGFBP-2; IGF-binding protein 2; insulin-like growth factor binding protein 2, 36kDa

products gene name :

IGFBP2

other gene names :

IGFBP2; IGFBP2; IBP2; IGF-BP53; BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP-2

uniprot entry name :

IBP2_HUMAN

reactivity :

Monkey

specificity :

This assay has high sensitivity and excellent specificity for detection of IGFBP-2. No significant cross-reactivity or interference between IGFBP-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IGFBP-2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This IGFBP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IGFBP-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cell Biology

products description :

Principle of the assay: IGFBP-2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IGFBP-2. Standards or samples are then added to the microtiter plate wells and IGFBP-2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IGFBP-2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IGFBP-2 are added to each well to "sandwich" the IGFBP-2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IGFBP-2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP-2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

290457647

ncbi acc num :

P18065.2

ncbi mol weight :

34,814 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); Myometrial Relaxation And Contraction Pathways (198759); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

uniprot summary :

IGFBP2: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Binds IGF2 more than IGF1. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2q35. Cellular Component: extracellular space; apical plasma membrane; extracellular region; cytoplasmic vesicle. Molecular Function: protein binding; insulin-like growth factor I binding; insulin-like growth factor II binding. Biological Process: response to drug; regulation of insulin-like growth factor receptor signaling pathway; response to retinoic acid; response to lithium ion; response to glucocorticoid stimulus; female pregnancy; positive regulation of activated T cell proliferation; signal transduction; response to estradiol stimulus; cellular response to hormone stimulus; cellular protein metabolic process; response to mechanical stimulus; regulation of cell growth; response to nutrient; aging

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage