catalog number :
MBS740443
products type :
ELISA Kit
products full name :
Rat Gamma Aminobutyric Acid ELISA Kit
products short name :
Gamma Aminobutyric Acid
other names :
gamma-aminobutyric acid
products gene name :
GABA
specificity :
This assay has high sensitivity and excellent specificity for detection of gamma-ABA. No significant cross-reactivity or interference between gamma-ABA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between gamma-ABA and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 nmol/mL.
products categories :
Neurobiology
products description :
Intended Uses This gamma-ABA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat gamma-ABA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: gamma-ABA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-gamma-ABA antibody and an gamma-ABA-HRP conjugate. The assay sample and buffer are incubated together with gamma-ABA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the gamma-ABA concentration since gamma-ABA from samples and gamma-ABA-HRP conjugate compete for the anti-gamma-ABA antibody binding site. Since the number of sites is limited, as more sites are occupied by gamma-ABA from the sample, fewer sites are left to bind gamma-ABA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The gamma-ABA concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAX31922.1